Cstps1
WebJul 8, 2024 · The proteins encoded by the full-length terpene synthase ( CsTPS1 ) and its alternative splicing isoform ( CsTPS1 - AS ) could catalyze the formation of geraniol when GPP was used as a substrate in vitro , whereas the expression of CsTPS1 - AS was only significantly induced by Colletotrichum gloeosporioides and Neopestalotiopsis sp. infection. CsTPS1 has previously been characterized as the key gene encoding the enzyme terpene synthase that catalyzes (+)-valencene biosynthesis (Sharon-Asa et al., 2003). Therefore, we screened a large set of citrus transcription factors for activation or inhibition using the promoter of CsTPS1 and a dual-luciferase assay ( Supplementary Fig. S1 ).
Cstps1
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WebOther disorders. One common alteration (polymorphism) in the CPS1 gene has been associated with increased risk of circulatory problems in newborns and in individuals who … WebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in …
WebMay 18, 2016 · The expression pattern of CitAP2.10 was positively correlated with (+)-valencene content and CsTPS1 expression. Dual-luciferase assays indicated that … WebNov 18, 2003 · Alignment of Cstps1 with tobacco and cotton sesquiterpene synthase‐deduced amino acid sequences.. Aligned sequences are of C. sinensis Cstps1 (this work), Nicotiana tabacum epi‐aristolochene synthase (Accession # T03714), and Gossypium arboreum cadinene synthase (Accession # CAA77191).Conserved sequence …
WebCsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa. The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 … WebDec 10, 2014 · The coding sequence of (+)-valencene synthase CsTPS1 ( C. sinensis) was obtained from NCBI GeneEntrez Database (GenBank: AF441124.1 ). The sequence was codon optimized for C. glutamicum by the Java Codon Adaptation Tool (JCat) ( Grote et al., 2005) and the optimized gene was synthesized by GeneArt ® life technologies™ …
WebOct 19, 2024 · CsTPS1-3 have predicted chloroplast-target peptides and produced predominantly linalool when GPP was supplied as substrate. Furthermore, CsTPS9 catalysed the formation of ( E )-β-ocimene and myrcene, and small amounts of sabinene and ( Z )-β-ocimene from GPP.
WebTwo recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. ‘Skunk’ trichome mRNA, have been isolated and characterized. Recombinant CsTPS1 showed a Km value of 6.8 μM, a Vmax of 1.1 x 10-4 µmol/min and V lithium refining stocks in indiaWebMar 1, 2007 · Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, ... ims bell roadWebCTP synthase 1. CTP synthase 1 is an enzyme that is encoded by the CTPS1 gene in humans. [5] [6] CTP synthase 1 is an enzyme in the de novo pyrimidine synthesis … lithium refining technologyWebCsTPS1- AS reduced the accumulation of geraniol, and the silenced tea plants exhibited greater susceptibility to pathogen infection than control plants. However, the geraniol content and pathogen resistance of CsTPS1-silenced plants and control plants did not signi cantly di er. Further analysis showed that silencing of CsTPS1- AS led to a ... lithium relaxed hairWebThe recombinant enzyme encoded by Cstps1 was shown to convert farnesyl diphosphate to a single sesquiterpene product identified as valencene by gas chromatography-mass spectrometry (GC-MS). Phylogenetic analysis of plant terpene synthase genes localized Cstps1 to the group of angiosperm sesquiterpene synthases. lithium release dateWebNon-small cell lung carcinoma (NSCLC) cells with oncogenic KRAS and loss of the tumor suppressor LKB1 express CPS1 and depend on this enzyme for growth. CPS1 maintains … ims bears com cnWebJun 1, 2004 · The characterization of the Cstps1 gene now provides researchers with an opportunity to engineer plants for high-level production of valencene, a highly valued flavor and fragrance compound. Adapted … ims belfort