Ctcf shrna
WebSep 1, 2016 · In CTCF-shRNA cells, CTCF expression was further diminished and PAX6, ADAM17 and RCN1 protein expressions were enhanced following the time course. Effects of altered CTCF activity on PAX6 and PAX6-associated ADAM17 and RCN1 expression were further measured by RT-PCR in both Lv-control and CTCF-shRNA HTCE cells. Web14 hours ago · All sgRNA and shRNA targeting sequences (Supplementary Tables ... the CTCF class targets were identified as sites co-occupied by 7 of 7 factors, ...
Ctcf shrna
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WebThe site navigation utilizes arrow, enter, escape, and space bar key commands. Left and right arrows move across top level links and expand / close menus in sub levels. WebNov 30, 2024 · 2. Results. We used shRNA knockdown to model the cellular consequences of reduced CTCF expression in K562 cells. Western blots showed that CTCF protein expression was significantly knocked down by ~80% in the presence of doxycycline (dox) in sh.CTCF K562 cells compared to non dox-treated cells and sh.control cells (Figure 1 …
WebJun 5, 2024 · CTCF shRNA and controls were analyzed by western blotting using anti-CTCF rabbit monoclonal antibody (Cell Signaling #3418) to verify knockdown. Cell … WebOct 1, 2012 · The 3C assay requires large numbers of cells in which CTCF has been knocked down, precluding the use of ex vivo pro-B cells. We therefore transduced a Rag2 −/− pro–B-cell line with retroviruses expressing control or CTCF shRNA. The cells were harvested 4 d after transduction and prepared for 3C analysis.
WebDec 26, 2024 · Efficient CTCF knockdown and single cell RNA-seq. We knocked down CTCF in EL4 cells by short hairpin RNA (shRNA). Western blotting showed a dramatic … WebApr 10, 2024 · In parallel, an unbiased in vivo shRNA screen to identify regulatory transcription factors of memory CD8 + T cell differentiation further confirmed the role of CTCF in CD8 + T cell differentiation (Figures S3B and S3C; Tables S4 and S5). shRNA constructs targeting CTCF were enriched in the T EM and T CM populations …
WebNov 30, 2024 · Inhibition of cell proliferation and clonogenicity following CTCF shRNA knockdown in K562 cells. (A) Immunoblot of CTCF shRNA knockdown in the presence and absence of doxycycline (dox) compared to ...
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