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Lamp2a knockout mouse

Webb7 juli 2024 · The role of chaperone-mediated autophagy (CMA) in cancer initiation and progression is not well understood due to the lack of a loss-of-function cancer models of LAMP2A, the key regulator of this process. Here, by generating an isoform-specific knockout of LAMP2A, we show that CMA deficiency promotes proliferation and tumor … Webb7 nov. 2024 · Lamp2 knockout (KO) mice are characterized by disrupted autophagy and phagocytosis in hepatocytes, neurons, and leukocytes (23–26). Loss-of-function …

Chaperone-mediated autophagy prevents cellular transformation …

Webb1 jan. 2024 · Since LAMP-2A is a critical molecular component of CMA, CRISPR-Cas9 gene-editing technology can be applied to accurately knock out LAMP-2A in human cancer cells without affecting the other two LAMP-2 isoforms that would provide a solid ground for thorough elucidation of CMA in tumorigenesis. WebbSynthetic peptide corresponding to Human LAMP2A aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 distinct isoforms, LAMP2A, … dividend\\u0027s j4 https://constancebrownfurnishings.com

条件性基因敲除小鼠 条件性基因敲除技术原理 Cre/loxP重组系统

WebbThe NIH-3T3 mouse embryonic fibroblast cells (American Type Culture Collection, CRL-1658™) were cultured in a 37°C incubator with 5% CO 2 in DMEM medium supplemented with 10% newborn calf serum (NCS). Mouse embryonic fibroblasts (MEFs) were generated from lamp2a null mice and corresponding wild-type littermates as described previously. Webb27 sep. 2024 · LAMP-2 Antibody (H4B4) is an IgG 1 κ mouse monoclonal LAMP-2 antibody (also designated LAMP2 antibody, LAMPB antibody or lysosomal associated membrane protein 2 antibody) suitable for the detection of the LAMP-2 protein of human origin by WB, IP, IF, IHC (P) and FCM. Webb1 apr. 2024 · In contrast, neurons from LAMP2A knockout mice presented alterations in protein trafficking, calcium homeostasis, and metabolism . Cellular energetics have been associated with CMA activity during the last decade ( 31 ). dividend\\u0027s j3

Transcription factor NFE2L2/NRF2 modulates chaperone-mediated …

Category:Loss of chaperone-mediated autophagy is associated with low

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Lamp2a knockout mouse

Chaperone-mediated Autophagy Deficiency Reprograms Cancer …

WebbGenetic and chemical inhibition of Lamp2A, a rate limiting factor of CMA, significantly blocked the loss of N-CoR in NSCLC cells, suggesting a crucial role of CMA in N-CoR degradation. ... Background Haptoglobin knockout (Hp-/-) mice are more sensitive to phenylhydrazine-induced hemolysis than Hp+/+ mice. Webb基因敲除小鼠和基因敲入小鼠：选赛业生物首创的基因敲除技术--TurboKnockout，ES打靶仅需6个月，零脱靶效应。服务类型有：完全性基因敲除小鼠、条件性基因敲除小鼠、人源化小鼠、基因敲入小鼠。

Lamp2a knockout mouse

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Webb31 jan. 2015 · To elucidate the neuronal function of LAMP-2 we analyzed knockout mice for neuropathological changes, MA and steady-state levels of CMA substrates. ... Cuervo AM, Dice JF (2000) Unique properties of lamp2a compared to other lamp2 isoforms. J Cell Sci 113(Pt 24):4441–4450. CAS PubMed Google Scholar ... Webb7 dec. 2024 · The results from this previous study showed significant LD accumulation in cultured mouse embryonic fibroblasts, as well as hepatic fat accumulation following LAMP2A knockout and are consistent with our observation that siRNA-mediated LAMP2A knockdown in hepatocytes also results in significant LD accumulation (SI Appendix, Fig. …

Webb1 jan. 2024 · Further studies will determine if stress-induced CMA upregulation in stellate cells contributes to the profibrotic phenotype of hepatocyte-specific Lamp2a knockout mice. Decreased protein quality control and subsequent lower resistance to stress in Lamp2a knockout mice [23] may also favor malignant transformation and liver … WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome.

Webb8 mars 2024 · Furthermore, an increased number and size of LDs and an accumulation of CE and TAG occurs in the liver from Lamp2A-knockout mouse. In addition, accumulation of TAG precursors, such as FFAs and DAG, is detected after 24 h of starvation, demonstrating an inadequate adaptation of the peripheral adipose tissue to the lipid … Webb15 okt. 2024 · LAMP2B was also expressed in fibroblasts and fetal liver. Northern blot analysis using LAMP2A-specific probes detected highest LAMP2A ... (2000) generated mice deficient in Lamp2 by targeted disruption. Lamp2 knockout mice showed elevated mortality and reduced weight compared with their wildtype littermates. About 50% of ...

WebbLAMP2A knockout results in a decreased CMA activity, which leads to increased PLIN2 expression, decreased lipid oxidation and subsequent LD accumulation [ 4, 6 ]. LAMP2A being the rate-limiting factor of CMA, its expression and/or stabilization of the protein is crucial to that process.

WebbLAMP2A localization in Ctns / cells but not Rab11 defects. Theup-regulationofRab11,Rab7,orRILP,butnotitstruncated form RILP-C33, rescued LAMP2A-defective trafficking in cys-tinosis, whereas dominant-negative Rab11 or Rab7 impaired LAMP2A trafficking. Treatment of cystinotic cells with a CMA activator increased … bebelWebbThe effect of LAMP2a-knockdown on tumor progression and TME maintaining was determined by using mouse tumor models. Findings: Lysosome associated membrane … dividend\\u0027s j9Webb10 juni 2024 · Similarly, the loss of chaperone-mediated autophagy through Lamp2A knockout (Lamp2A flox/flox; Alb-Cre) also promotes the development of hepatocellular adenomas with increasing age []. Yet whilst the loss of autophagic activity can drive the initial formation of tumorigenesis, this is limited to benign adenomas, and no malignant … dividend\\u0027s j8Webb1 apr. 2024 · Western blot analysis of LAMP2a expression in JAR cell lysate (MBS179696). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V … bebel 13Webb20 aug. 2024 · 最后，让这两只小鼠进行交配，产生的同时含有上述两种基因型的子代小鼠就会在某一特定类型的细胞中缺失某一特定的基因（图3）。. 4. Cre/loxP重组系统优势及问题. 目前，Cre-loxP系统是应用最广泛的 条件性基因敲除工具 ，主要因其因有如下优 … dividend\\u0027s jnWebb24 apr. 2024 · 溶酶体上的LAMP2A受体越多，潜在的CMA活动水平就越高。 这种名为CA的新型药物通过增加这些LAMP2A受体的数量而发挥作用。 这些作者表示，人们在一生中产生相同数量的LAMP2A受体，不过，这些受体随着年龄的增加而退化得更快，因此老年人往往有较少的受体用于将不需要的蛋白递送到溶酶体。 CA将LAMP2A恢复到年轻时 … bebel acnhWebbImmunohistochemical analysis of paraffin-embedded Mouse liver labeling LAMP2A with unpurified ab125068 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at … bebel adalah